LOCUS       40924_42939        3052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTD1(TM) DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Suzuki T, Ito M, Ezure T, Kobayashi S, Shikata M, Tanimizu K, 
            Nishimura O.
  TITLE     Performance of expression vector, pTD1, in insect cell-free 
            translation system
  JOURNAL   J. Biosci. Bioeng. 102 (1), 69-71 (2006)
  PUBMED    16952840
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Suzuki T, Shikata M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2004) Masamitsu Shikata, SHIMADZU CORPORATION, 
            Analytical & Measuring Instruments Division, Life Science 
            Laboratory; 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, Kyoto 
            604-8511, Japan (E-mail:mshikata@shimadzu.co.jp, 
            URL:http://www.shimadzu.com, Tel:81-75-823-1351, Fax:81-75-823-1368)
REFERENCE   3  (bases 1 to 3052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci. 
            Bioeng."; date: "2006"; volume: "102"; issue: "1"; pages: "69-71"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-NOV-2004) Masamitsu Shikata, SHIMADZU CORPORATION, Analytical ";
            volume: " 1, Nishinokyo-Kuwabaracho, Nakagyo-ku, Kyoto, Kyoto 
            604-8511, Japan (E-mail:mshikata@shimadzu.co.jp, 
            URL:http://www.shimadzu.com, Tel:81-75-823-1351, Fax"; pages: 
            "81-75-823-1368"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           259..304
                     /label=polyhedrin 5' UTR
                     /note="5' UTR from the Malacosoma neustria
                     nucleopolyhedrovirus polyhedrin gene (Ezure et al., 2006)"
     misc_feature    305..342
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     3'UTR           359..731
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     misc_feature    736..757
                     /label=synthetic poly(A)20 region
                     /note="synthetic poly(A)20 region"
     terminator      758..805
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(831..847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(855..871)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(879..909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(924..945)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1233..1821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1995..2852)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2853..2957)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcctt
      241 aatacgactc actataggag tatttttatt ctttcgtaaa aaaattagaa aaataaaata
      301 taaagatatc gaattcgagc tcggtacccg gggatcctct agagtcgggc ggctgtaaaa
      361 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
      421 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
      481 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
      541 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
      601 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
      661 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
      721 caaaatatta tccgcaaaaa aaaaaaaaaa aaaaaaacta gcataacccc ttggggcctc
      781 taaacgggtc ttgaggggtt ttttgcggcc gcaagcttgg cgtaatcatg gtcatagctg
      841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      961 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1021 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1141 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1201 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1261 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1681 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1801 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1861 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1921 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1981 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2041 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2101 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2161 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2221 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2281 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2341 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2401 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2461 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2521 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2581 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2641 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2701 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2761 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2821 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2881 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2941 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3001 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
